RABBIT ANTI HUMAN CD184 (N-TERMINAL) | MyBioSource MBS221580 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN CD184 (N-TERMINAL)

catalog :

MBS221580

quantity :

0.1 mg

price :

380 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221580

products type :

Antibody

products full name :

RABBIT ANTI HUMAN CD184 (N-TERMINAL)

products short name :

CD184

other names :

C-X-C chemokine receptor type 4 isoform a; C-X-C chemokine receptor type 4; C-X-C chemokine receptor type 4; CD184 antigen; LPS-associated protein 3; SDF-1 receptor; fusin; leukocyte-derived seven transmembrane domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; chemokine (C-X-C motif) receptor 4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide-associated protein 3; LAP-3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD_antigen: CD184

products gene name :

CD184

other gene names :

CXCR4; CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E; CXC-R4; CXCR-4; LESTR; LAP-3; LPS-associated protein 3; SDF-1 receptor

uniprot entry name :

CXCR4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

356

specificity :

This item recognises the human G-protein-coupled chemokine receptor CD184 (CXCR4), a 40kDa cell surface protein known to act as a co-factor in HIV infection of CD4+ T cells. Mutations in the CD184 gene are associated with WHIM syndrome, an inherited combined immunodeficiency disease, see Taniuchi, S. et al. for details.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 0.5 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

app notes :

Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by ion exchange chromatography

other info2 :

Immunogen: Peptide sequence corresponding to amino acids 1-14 of human CD184. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human CD184 were raised by repeated immunisation of rabbits with highly purified antigen. Target Species: Human

products description :

Rabbit anti Human CD184 antibody recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40kDa cell surface protein known to act as a co-factor in HIV infection of CD4+ T cells (Bleul et al. Mutations in the CD184 gene are associated with WHIM syndrome, an inherited combined immunodeficiency disease (Taniuchi et al. ).

ncbi gi num :

56790927

ncbi acc num :

NP_001008540.1

ncbi gb acc num :

NM_001008540.1

uniprot acc num :

P61073

ncbi mol weight :

40,221 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Binding And Entry Of HIV Virion Pathway (105986); CXCR4-mediated Signaling Events Pathway (137910); Cardiac Progenitor Differentiation Pathway (712094); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051)

ncbi summary :

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

CXCR4: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Monomer. Can form dimers. Interacts with CD164. Interacts with HIV-1 surface protein gp120 and Tat. Interacts with ARRB2; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and allows activation of MAPK1 and MAPK3. Interacts with ARRC; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and modulates calcium mobilization. Interacts (via the cytoplasmic C-terminal) with ITCH (via the WW domains I and II); the interaction, enhanced by CXCL12, ubiquitinates CXCR4 and leads to its degradation. Interacts with extracellular ubiquitin. Interacts with human cytomegalovirus/HHV- 5 protein UL78. Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q21. Cellular Component: cell surface; lysosome; leading edge; cytoplasmic membrane-bound vesicle; early endosome; integral to membrane; intercellular junction; growth cone; late endosome; cytoplasm; plasma membrane; cytoplasmic vesicle; external side of plasma membrane. Molecular Function: viral receptor activity; G-protein coupled receptor activity; protein binding; ubiquitin binding; cytokine binding; ubiquitin protein ligase binding; myosin light chain binding; coreceptor activity; actin binding; C-X-C chemokine receptor activity. Biological Process: entry of virus into host cell; regulation of chemotaxis; viral reproduction; apoptosis; activation of MAPK activity; neuron migration; motor axon guidance; regulation of cell migration; germ cell development; T cell proliferation; elevation of cytosolic calcium ion concentration; germ cell migration; dendritic cell chemotaxis; positive regulation of oligodendrocyte differentiation; inflammatory response; neutrophil activation; response to virus; calcium-mediated signaling; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; ameboidal cell migration; myelin maintenance; response to hypoxia; entry into host cell; brain development. Disease: Whim Syndrome

size1 :

0.1 mg

price1 :

380 USD

more info or order :

MyBioSource product webpage