RABBIT ANTI HUMAN RANTES | MyBioSource MBS221559 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI HUMAN RANTES

catalog :

MBS221559

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, enzyme immunoassay, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221559

products type :

Antibody

products full name :

RABBIT ANTI HUMAN RANTES

products short name :

RANTES

other names :

C-C motif chemokine 5 isoform 1; C-C motif chemokine 5; C-C motif chemokine 5; T-cell specific protein p288; beta-chemokine RANTES; eosinophil chemotactic cytokine; regulated upon activation, normally T-expressed, and presumably secreted; small inducible cytokine subfamily A (Cys-Cys), member 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTESCleaved into the following 2 chains:RANTES(3-68); RANTES(4-68)

other gene names :

CCL5; CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta; D17S136E; SCYA5; TCP228

uniprot entry name :

CCL5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0 mg/ml after reconstitution

storage stability :

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)

app notes :

ELISA: This purified human RANTES antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human RANTES antibody as the detection reagent and recombinant human RANTES as the standard. Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES as the positive control. ELISA: Minimum Dilution: 0.5 ug/ml; Maximum Dilution: 2.0ug/ml. Functional Assays: Minimum Dilution: 5.0ug/ml; Maximum Dilution: 7.0ug/ml. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

other info2 :

Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography. Target Species: Human

products description :

Rabbit anti Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells. RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

ncbi gi num :

22538814

ncbi acc num :

NP_002976.2

ncbi gb acc num :

NM_002985.2

uniprot acc num :

P13501

ncbi mol weight :

9,990 Da

ncbi pathways :

Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytosolic DNA-sensing Pathway 125137!!Cytosolic DNA-sensing Pathway 124833

ncbi summary :

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

size :

0.1 mg

price :

540 USD

more info or order :

MyBioSource product webpage