antibody

RABBIT ANTI HUMAN Chk2 (pThr68)

MBS221538

0.05 mg

475 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, chimpanzee

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI HUMAN Chk2 (pThr68)

Chk2

serine/threonine-protein kinase Chk2 isoform c; Serine/threonine-protein kinase Chk2; serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; cds1 homolog; checkpoint-like protein CHK2; checkpoint kinase 2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Chk2

CHEK2; CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; CDS1; CHK2; RAD53; Hucds1; hCds1

CHK2_HUMAN

Polyclonal

IgG

Rabbit

Chimpanzee (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)

586

This item detects human Checkpoint kinase 2 (Chk2), phosphorylated at threonine 68 (Thr68). Chk2 is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radiation, and phsophorylate Thr68 and other sites in the amino-terminal region of Chk2, leading to Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, preventing the entry into mitosis, and has been shown to stabilize the tumour suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumour suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familial cancer usually associated with inherited mutations in TP53.

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)*

Western Blot: This item detects a band of approximately 61kDa. ELISA: Minimum Dilution: 1/10000; Maximum Dilution: 1/50000. Western Blotting: Minimum Dilution: 1/200; Maximum Dilution: 1/2000; Application Note: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Perservative Stabilisers: 0.01% Sodium Azide (NaN3)

Immunogen: Synthetic peptide corresponding to amino acids 64-73 of human Chk2. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to Chk2 (pTHR68) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Human

MBS221538 detects human Checkpoint kinase 2 (Chk2), phosphorylated at threonine 68 (Thr68). Chk2 is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radiation, and phsophorylate Thr68 and other sites in the amino-terminal region of Chk2, leading to Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, preventing the entry into mitosis, and has been shown to stabilize the tumour suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumour suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familial cancer usually associated with inherited mutations in TP53.

54112407

NP_001005735.1

NM_001005735.1

O96017

36,157 Da

Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!DNA Damage Response Pathway 198788!!DNA Damage-induced Cell Cycle Checkpoints Pathway 1084767!!DNA Damage-induced Cell Cycle Checkpoints Pathway 1108230!!FOXM1 Transcription Factor Network Pathway 137935!!G1/S DNA Damage Checkpoints Pathway 105740

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

0.05 mg

475 USD