antibody

RABBIT ANTI HUMAN AURORA-B KINASE

MBS221534

0.05 mg

490 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, cow, chimpanzee

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI HUMAN AURORA-B KINASE

AURORA-B KINASE

aurora kinase B isoform 2; Aurora kinase B; aurora kinase B; ARK-2; STK-1; aurora 1; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora- and Ipl1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-related kinase 2; aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine-protein kinase 12; serine/threonine-protein kinase 5; serine/threonine-protein kinase aurora-B; aurora kinase B; Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B

AURKB; AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2; AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2; Aurora-related kinase 2

AURKB_HUMAN

Polyclonal

IgG

Rabbit

Bovine (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence)

303

This item detects Aurora-B kinase (AurB), a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

Purified. Purified IgG - liquid

IgG concentration 0.2mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)

Western Blot: This item detects a band of approximately 39kDa in HeLa cell lysates. ELISA: Minimum Dilution: 1/2000; Maximum Dilution: 1/10000. Western Blotting: Minimum Dilution: 1/250; Maximum Dilution: 1/1000

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)

Immunogen: Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to human Aurora-B kinase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography. Target Species: Human

MBS221534 detects Aurora-B kinase (AurB), a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

378786658

NP_001243763.1

NM_001256834.1

Q96GD4

39,467 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!Aurora A Signaling Pathway 137925!!Aurora B Signaling Pathway 138080!!Aurora C Signaling Pathway 138003!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!FOXM1 Transcription Factor Network Pathway 137935!!M Phase Pathway 105812!!Mitotic Anaphase Pathway 105818

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

0.05 mg

490 USD