RABBIT ANTI SOCS-1 (C-TERMINAL) | MyBioSource MBS221493 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI SOCS-1 (C-TERMINAL)

catalog :

MBS221493

quantity :

0.1 mg

price :

335 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221493

products type :

Antibody

products full name :

RABBIT ANTI SOCS-1 (C-TERMINAL)

products short name :

SOCS-1

other names :

suppressor of cytokine signaling 1; Suppressor of cytokine signaling 1; suppressor of cytokine signaling 1; JAK binding protein; JAK-binding protein; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; TIP-3; Tec-interacting protein 3; cytokine-inducible SH2 protein 1; suppressor of cytokine signaling 1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3

products gene name :

SOCS-1

other gene names :

SOCS1; SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1; SSI1; TIP3; SOCS-1; JAB; SSI-1; TIP-3

uniprot entry name :

SOCS1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat

sequence length :

211

specificity :

This item recognises Suppressor of cytokine signalling 1 (SOCS1), a 24kDa member of the Suppressor of cytokine signalling family. SOCS1 acts downstream of cytokine receptors and plays a role in the negative regulation of cytokine signalling. It acts mainly on the Janus kinase (JAK)/signal transducers and activators of transcription-3 (STAT3) pathway but also inhibits signalling by cytokines such as insulin and toll-like receptors. Furthermore SOCS1 is involved in ubiquitin-dependent protein degradation, plays a role in T cell activation and in the regulation of IL-6, IL-4, IL-12 and IL-15. SOCS1 is expressed ubiquitously and at elevated levels in the spleen, small intestine and peripheral blood leukocytes.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Western Blot (WB)

app notes :

Western Blot: This item detects a bands of approximately 1.0-23.0ug/ml in human spleen cell lysates. Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml

other info1 :

Perservative Stabilisers: 0.02% Sodium Azide. Preparation: Antisera to human SOCS1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

other info2 :

Immunogen: A 15 amino acid peptide located near the carboxy terminus of human SOCS1. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

MBS221493 recognises Suppressor of cytokine signalling 1 (SOCS1), a 24kDa member of the Suppressor of cytokine signalling family. SOCS1 acts downstream of cytokine receptors and plays a role in the negative regulation of cytokine signalling. It acts mainly on the Janus kinase (JAK)/signal transducers and activators of transcription-3 (STAT3) pathway but also inhibits signalling by cytokines such as insulin and toll-like receptors. Furthermore SOCS1 is involved in ubiquitin-dependent protein degradation, plays a role in T cell activation and in the regulation of IL-6, IL-4, IL-12 and IL-15. SOCS1 is expressed ubiquitously and at elevated levels in the spleen, small intestine and peripheral blood leukocytes.

ncbi gi num :

4507233

ncbi acc num :

NP_003736.1

ncbi gb acc num :

NM_003745.1

uniprot acc num :

O15524

ncbi mol weight :

23,551 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); ECS Complex Pathway (413455); ECS Complex Pathway (468394); EGFR1 Signaling Pathway (198782); EPO Receptor Signaling Pathway (198882)

ncbi summary :

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]

uniprot summary :

SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 16p13.13. Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; nucleus; cytosol. Molecular Function: insulin-like growth factor receptor binding; protein binding; protein kinase inhibitor activity; kinase inhibitor activity; protein kinase binding. Biological Process: fat cell differentiation; regulation of protein amino acid phosphorylation; regulation of growth; cytokine and chemokine mediated signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; JAK-STAT cascade; negative regulation of JAK-STAT cascade

size1 :

0.1 mg

price1 :

335 USD

more info or order :

MyBioSource product webpage