This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
SHEEP ANTI 14-3-3 (pSer185)
catalog :
MBS221344
quantity :
0.1 mg
price :
455 USD
clonality :
polyclonal
host :
sheep
conjugate :
nonconjugated
application :
western blot
product information
catalog number :
MBS221344
products type :
Antibody
products full name :
SHEEP ANTI 14-3-3 (pSer185)
products short name :
14-3-3
other names :
14-3-3 protein gamma; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1
products gene name :
14-3-3
other gene names :
YWHAG; KCIP-1
uniprot entry name :
1433G_SHEEP
clonality :
Polyclonal
isotype :
IgG
host :
Sheep
reactivity :
Bovine, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat
sequence length :
158
specificity :
This item recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from the date of despatch.
tested application :
Western Blot (WB)*
app notes :
Western Blot: This item detects a band of approximately 30kDa. Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/300; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
other info2 :
Immunogen: Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC. Antiserum Preparation: Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Sheep
products description :
MBS221344 recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
ncbi gi num :
71153782
ncbi acc num :
P68253.1
ncbi mol weight :
18,000 Da
size :
0.1 mg
price :
455 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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