RABBIT ANTI RAT MCP-1 | MyBioSource MBS221294 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RABBIT ANTI RAT MCP-1

catalog :

MBS221294

quantity :

0.1 mg

price :

540 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS221294

products type :

Antibody

products full name :

RABBIT ANTI RAT MCP-1

products short name :

MCP-1

other names :

C-C motif chemokine 2; C-C motif chemokine 2; C-C motif chemokine 2; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic protein 1; small inducible cytokine A2; small inducible gene JE; small-inducible cytokine A2; chemokine (C-C motif) ligand 2; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2

products gene name :

MCP-1

other gene names :

Ccl2; Ccl2; MCP-1; Scya2; Sigje; Je; Mcp1; Scya2; MCP-1

uniprot entry name :

CCL2_RAT

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

sequence length :

148

specificity :

This antibody recognizes rat monocyte chemo attractant protein-1 (MCP-1) also know as CCL-2. Rat MCP-1 is a chemokine of approximately 12kD, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

form :

Purified. Purified IgG - lyophilised

concentration :

IgG concentration 1.0 mg/ml after reconstitution.

storage stability :

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

app notes :

ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;. Functional Assays: Minimum Dilution: 4.0; Maximum Dilution: 6.0ug/ml;. Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

other info1 :

Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide

other info2 :

Immunogen: Recombinant rat MCP-1 (PRP17). Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. Target Species: Rat

products description :

Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2. Rat MCP-1 is a chemokine of approximately 12kD, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

ncbi gi num :

13928714

ncbi acc num :

NP_113718.1

ncbi gb acc num :

NM_031530.1

uniprot acc num :

P14844

ncbi mol weight :

16,460 Da

ncbi pathways :

Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1120716); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1120714); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1122016); Disease Pathway (1121849)

ncbi summary :

a monocyte chemoattractant protein [RGD, Feb 2006]

uniprot summary :

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family. Protein type: Chemokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Cellular Component: extracellular space; rough endoplasmic reticulum; cell soma; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal. Molecular Function: heparin binding; G-protein-coupled receptor binding; CCR2 chemokine receptor binding; chemokine activity. Biological Process: positive regulation of cell adhesion; maternal process involved in pregnancy; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; regulation of cell shape; monocyte chemotaxis; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; response to amino acid stimulus; positive regulation of tumor necrosis factor production; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; positive regulation of cell-cell adhesion; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to wounding; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

size1 :

0.1 mg

price1 :

540 USD

more info or order :

MyBioSource product webpage