antibody

RABBIT ANTI GLUCOSE TRANSPORTER 4

MBS220873

0.1 mL

475 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunoprecipitation

Antibody

RABBIT ANTI GLUCOSE TRANSPORTER 4

GLUCOSE TRANSPORTER 4

solute carrier family 2, facilitated glucose transporter member 4; Solute carrier family 2, facilitated glucose transporter member 4; solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; solute carrier family 2 (facilitated glucose transporter), member 4; Glucose transporter type 4, insulin-responsive; GLUT-4

SLC2A4; SLC2A4; GLUT4; GLUT4; GLUT-4

GTR4_HUMAN

Polyclonal

IgG

Rabbit

Monkey, Mouse, Rat

509

This item recognizes glucose transporter 4 (GLUT4). It does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin.GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Purified. Purified IgG - liquid

Approximate Protein Concentration: 5.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)

Immunohistology - Paraffin: Minimum Dilution: 10; Maximum Dilution: 20 ug/ml

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography

Immunogen: A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin. Buffer Solution: Phosphate buffered saline. Target Species: Human

MBS220873 recognizes glucose transporter 4 (GLUT4). It does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin.GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

4507011

NP_001033.1

NM_001042.2

P14672

43,760 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Arf6 Trafficking Events Pathway (137954); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Developmental Biology Pathway (477129); Disease Pathway (530764)

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

GLUT4: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is regulated by insulin. Has two internalization sequences, a dileucine repeat present in the C-terminus and a FxxY motif in the amino-terminal end. Associates with an intracellular tubulo-vesicular compartment under low plasma conditions. The binding of insulin to the insulin receptor (a typosine kinase) leads to the rapid translocation of GLUT4 to the cell surface, increasing cellular glucose transport activity. Its translocation to the plasma membrane is also stimulated by exercise, but independent from insulin signaling. This insulin-independent pathway may be regulated by AMPK. Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: clathrin-coated vesicle; integral to plasma membrane; T-tubule; endomembrane system; coated pit; vesicle membrane; cytosol; lipid raft; multivesicular body; trans-Golgi network transport vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: D-glucose transmembrane transporter activity; protein binding; glucose transmembrane transporter activity. Biological Process: amylopectin biosynthetic process; response to ethanol; cellular response to insulin stimulus; hexose transport; glucose import; carbohydrate metabolic process; brown fat cell differentiation; pathogenesis; glucose transport; glucose homeostasis; transmembrane transport. Disease: Diabetes Mellitus, Noninsulin-dependent

0.1 mL

475 USD