catalog number :
MBS220763
products full name :
RABBIT ANTI MOUSE COLLAGEN IV
products short name :
COLLAGEN IV
other names :
Col4a5 protein; Col4a5 protein; Col4a5 protein
other gene names :
Col4a5
uniprot entry name :
Q80V57_MOUSE
reactivity :
Orangutan, Rat
form :
Purified. Purified Ig - liquid
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin
app notes :
ELISA: Minimum Dilution: 1/100; Maximum Dilution: 1/400;. Immunofluorescence: Minimum Dilution: 1/40; Maximum Dilution: 1/160;. Immunohistology - Paraffin: Minimum Dilution: 1/250; Maximum Dilution: 1/1000
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by antigen column chromatography
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Mouse
products description :
Rabbit amti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28kDa arresten fragment, collagen alpha2(IV) yields a c-terminal canstatin fragment while Collagen alpha3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9). Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007). Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (e.g. Tang et al.), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013). The following cross reactivities have been observed:Mouse type IV100%Mouse types I, II & III
ncbi mol weight :
58,283 Da