antibody

RABBIT ANTI CHICKEN INTERFERON GAMMA:Preservative Free

MBS220076

0.5 mg

550 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

RABBIT ANTI CHICKEN INTERFERON GAMMA:Preservative Free

IFN GAMMA

interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; Interferon-gamma level; interferon-gama; interferon, gamma

IFNG; IFNG; IFN-gamma

IFNG_CHICK

Polyclonal

IgG

Rabbit

164

This item recognizes chicken Interferon gamma (chIFN-gamma). Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFN-gamma strongly upregulates the expression of MHC class II and iNOS genes. This item, can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta, see Weining, K.C. et al. for details.

Preservative Free. Purified IgG - liquid

IgG concentration 0.5 mg/ml

Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

Western Blot: This item detects a band of approximately 18 kDa.

Immunogen: Recombinant chicken Interferon Gamma. Buffer Solution: Phosphate buffered saline. Antiserum Preparation: Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G. Target Species: Chicken

MBS220076 recognizes chicken Interferon gamma (chIFN-gamma). Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFN-gamma strongly upregulates the expression of MHC class II and iNOS genes. MBS220076, can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta, see Weining, K.C. et al. for details.

45433518

NP_990480.1

NM_205149.1

P49708

18,953 Da

Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Herpes Simplex Infection Pathway (377875); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217175); Influenza A Pathway (217150); Jak-STAT Signaling Pathway (83972); Jak-STAT Signaling Pathway (488); Proteasome Pathway (83949); Proteasome Pathway (445)

0.5 mg

550 USD