GOAT ANTI HUMAN IgM:Alk. Phos. | MyBioSource MBS220006 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GOAT ANTI HUMAN IgM:Alk. Phos.

catalog :

MBS220006

quantity :

1 mL

price :

440 USD

clonality :

polyclonal

host :

goat

conjugate :

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS220006

products type :

Antibody

products full name :

GOAT ANTI HUMAN IgM:Alk. Phos.

products short name :

IgM

other names :

Ig mu chain C region, partial; Ig mu chain C region; immunoglobulin heavy constant mu

products gene name :

IgM

other gene names :

IGHM; IGHM; MU; VH; AGM1

uniprot entry name :

IGHM_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

sequence length :

452

form :

Alk. Phos. Purified IgG conjugated to Alkaline Phosphatase - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from the date of despatch.

tested application :

Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB)

app notes :

ELISA: Minimum Dilution: 1/2000; Maximum Dilution: 1/4000

other info1 :

Perservative Stabilisers: 0.1% Sodium Azide. 50% Glycerol

other info2 :

Buffer Solution: TRIS buffered saline, 1mM MgCl2. Antiserum Preparation: Antiserum to human IgM was raised by repeated immunisation of goats with human IgM paraproteins. The preparation was purified by affinity chromatography on human IgM covalently linked to agarose. Target Species: Human

products description :

Goat anti human IgM polyclonal antibody reacts with the heavy chain of human IgM. This antibody has been cross-absorbed to reduce cross-reactivity with human IgG and IgA, but may react with IgM from other species.

ncbi gi num :

193806374

ncbi acc num :

P01871.3

uniprot acc num :

P01871

ncbi mol weight :

51,790 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); Immune System Pathway (106386); Signaling By The B Cell Receptor (BCR) Pathway (576248)

ncbi summary :

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]

uniprot summary :

IGHM: IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane- bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as an homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts. Defects in IGHM are the cause of agammaglobulinemia type 1 (AGM1). It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 14q32.33. Cellular Component: extracellular space; cell surface; plasma membrane; integral to membrane. Molecular Function: peptidoglycan binding; phosphatidylcholine binding; antigen binding; single-stranded DNA binding. Biological Process: adaptive immune response; antibacterial humoral response; innate immune response; defense response to Gram-negative bacterium. Disease: Agammaglobulinemia 1, Autosomal Recessive

size1 :

1 mL

price1 :

440 USD

more info or order :

MyBioSource product webpage