HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4 | MyBioSource MBS219861 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4

catalog :

MBS219861

quantity :

0.25 mg

price :

365 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

HMD4-2

reactivity :

mouse

application :

flow cytometry, FACS

product information

catalog number :

MBS219861

products type :

Antibody

products full name :

HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4

products short name :

DELTA-LIKE PROTEIN 4

other names :

delta-like protein 4; Delta-like protein 4; delta-like protein 4; drosophila Delta homolog 4; delta-like 4 (Drosophila); Drosophila Delta homolog 4; Delta4

other gene names :

Dll4; Dll4; Delta4; Delta4

uniprot entry name :

DLL4_MOUSE

clonality :

Monoclonal

isotype :

IgG

clone :

HMD4-2

host :

Hamster

sequence length :

686

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunohistology Paraffin*

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G

other info2 :

Immunogen: Recombinant mouse DLL4. Histology Positive Control Tissue: Mouse spleen. Fusion Partners: Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Mouse

products description :

Hamster anti Mouse Delta-Like Protein 4 antibody, clone HMD4-2 specifically recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumour growth by promoting non-productive angiogenesis. Hamster anti Mouse Delta-Like Protein 4 ant

ncbi gi num :

244799228

ncbi acc num :

NP_062327.2

ncbi gb acc num :

NM_019454.3

uniprot acc num :

Q9JI71

ncbi mol weight :

74,991 Da

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway 1110490!!Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway 1111366!!Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway 1111370!!Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway 1111368!!Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway 1111364!!Delta-Notch Signaling Pathway 198316!!Disease Pathway 1111319!!FBXW7 Mutants And NOTCH1 In Cancer Pathway 1111371!!NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway 1110493!!Notch Signaling Pathway 198301

size :

0.25 mg

price :

365 USD