catalog number :
MBS219528
products full name :
MOUSE ANTI NITROTYROSINE
products short name :
NITROTYROSINE
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
app notes :
ELISA: Minimum Dilution: 2; Maximum Dilution: 100ng/ml;. Immunoprecipitation: Minimum Dilution: 1.0ug/ml;. Western Blotting: Minimum Dilution: 0.1ug/ml
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein A
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Chemical
products description :
Mouse anti Nitrotyrosine antibody, clone 6B2-3G2 recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro haemoglobin. Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, inflammatory bowel disease and cystic fibrosis (Halliwell 1997). The specificity of Mouse anti Nitrotyrosine antibody, clone 6B2-3G2 as determined by competition ELISA is: Nitrotyrosine:95 % of inhibitionTyrosine:0 % of inhibitionNitrotryptophan:4 % of inhibitionAminotyrosine:0 % of inhibitionPhosphotyrosine:2 % of inhibitionChlorotyrosine:3 % of inhibition