MyBioSource

antibody

MOUSE ANTI HUMAN FACTOR VIII

MBS219502

0.2 mg

415 USD

monoclonal

mouse

human

enzyme immunoassay, western blot, radioimmunoassay, ELISA

MBS219502

Antibody

MOUSE ANTI HUMAN FACTOR VIII

FACTOR VIII

coagulation factor VIII isoform a; Coagulation factor VIII; coagulation factor VIII; antihemophilic factor; coagulation factor VIIIc; factor VIII F8B; coagulation factor VIII, procoagulant component; Antihemophilic factor; AHF; Procoagulant componentCleaved into the following 4 chains:Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain

F8; F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E; F8C; AHF

FA8_HUMAN

Monoclonal

IgG1

RFF-VIIIC/5

Mouse

2351

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Radioimmunoassays (RIA), Western Blot (WB)

ELISA: Minimum Dilution: 1/400; Maximum Dilution: 1/4000

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Affinity purified human Factor VIII. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes an epitope towards the C-terminus of full-length Factor VIII. It also recognizes the 80kDa and 70kDa cleavage products.

4503647

NP_000123.1

NM_000132.3

P00451

24,641 Da

Blood Clotting Cascade Pathway 198840!!Common Pathway 106060!!Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!Hemostasis Pathway 106028!!Intrinsic Pathway 106059!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]

0.2 mg

415 USD