RAT ANTI MOUSE P2X7 | MyBioSource MBS219463 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI MOUSE P2X7

catalog :

MBS219463

quantity :

0.1 mg

price :

205 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

Hano43

reactivity :

mouse

application :

immunocytochemistry, flow cytometry, FACS

product information

catalog number :

MBS219463

products type :

Antibody

products full name :

RAT ANTI MOUSE P2X7

products short name :

P2X7

other names :

P2X purinoceptor 7 isoform a; P2X purinoceptor 7; P2X purinoceptor 7; ATP receptor; P2X7 purinoceptor; P2X7 receptor; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; ATP receptor; P2Z receptor; Purinergic receptor

products gene name :

P2X7

other gene names :

P2rx7; P2rx7; P2X7R; P2X(7); AI467586; P2x7; P2X7

uniprot entry name :

P2RX7_MOUSE

clonality :

Monoclonal

isotype :

IgG2b

clone :

Hano43

host :

Rat

sequence length :

595

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS), Immunofluorescence (IF)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Immunogen: A P2X7-expression construct and a final boost with P2X7-transfected HEK cells. Fusion Partners: Spleen cells from immunised rats were fused with cells of the Sp2/0 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Mouse

products description :

Rat anti Mouse P2X7 antibody, clone Hano43 recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.

ncbi gi num :

84781766

ncbi acc num :

NP_035157.2

ncbi gb acc num :

NM_011027.3

uniprot acc num :

Q9Z1M0

ncbi mol weight :

68,388 Da

ncbi pathways :

Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Immune System Pathway (1110668); Inflammasomes Pathway (1110742); Innate Immune System Pathway (1110697); Neuroactive Ligand-receptor Interaction Pathway (83250); Neuroactive Ligand-receptor Interaction Pathway (462); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (1110740); The NLRP3 Inflammasome Pathway (1110743)

uniprot summary :

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation. Protein type: Receptor, misc.; Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral. Cellular Component: cell soma; membrane; integral to plasma membrane; plasma membrane; integral to membrane; synapse; integral to nuclear inner membrane; intercellular junction; intracellular; neuromuscular junction; cytosol; external side of plasma membrane. Molecular Function: protein binding; lipopolysaccharide binding; channel activity; ion channel activity; receptor activity; purinergic nucleotide receptor activity; ATP binding; ATP-gated cation channel activity. Biological Process: positive regulation of catalytic activity; activation of MAPK activity; positive regulation of apoptosis; positive regulation of T cell mediated cytotoxicity; cytolysis; programmed cell death; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; response to organic cyclic substance; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; multicellular organismal protein catabolic process; ceramide biosynthetic process; T cell homeostasis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; protein oligomerization; phospholipid translocation; phagolysosome formation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to bacterium; positive regulation of protein secretion; response to mechanical stimulus; response to zinc ion; cell volume homeostasis; response to calcium ion; phospholipid transfer to membrane; cell morphogenesis; response to organic substance; T cell proliferation; positive regulation of MAPKKK cascade; positive regulation of mitochondrial depolarization; plasma membrane organization and biogenesis; transport; calcium ion transport; pore complex biogenesis; negative regulation of bone resorption; inflammatory response; cation transport; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; positive regulation of ossification; positive regulation of interleukin-1 beta production; synaptic vesicle exocytosis; homeostasis of number of cells within a tissue; skeletal morphogenesis; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; protein processing; gene expression; ion transport; positive regulation of protein amino acid phosphorylation; membrane budding; positive regulation of cytokine secretion

size1 :

0.1 mg

price1 :

205 USD