catalog number :
MBS219414
products full name :
MOUSE ANTI BOVINE CD14:FITC
products short name :
CD14
other names :
monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein; CD14 molecule<; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
products gene name :
CD14
other gene names :
CD14; CD14; N/A
uniprot entry name :
CD14_BOVIN
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Bovine
products description :
Mouse anti Bovine CD14, clone CC-G33 is a monoclonal antibody recognizing bovine CD14, a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells, and also recognises an epitope on ovine CD14, see Sopp et al. 1996 for details. CC-G33 has also been shown to be reactive with CD14 from the Water buffalo (Bubalus bubalis), see Mirielli et al. 2013.
ncbi acc num :
NP_776433.1
ncbi gb acc num :
NM_174008.1
ncbi mol weight :
39,667 Da
ncbi pathways :
Activated TLR4 Signalling Pathway 1119352!!Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway 1119338!!Amoebiasis Pathway 167315!!Amoebiasis Pathway 167191!!Hematopoietic Cell Lineage Pathway 84317!!Hematopoietic Cell Lineage Pathway 489!!IKK Complex Recruitment Mediated By RIP1 Pathway 1119339!!Immune System Pathway 1119286!!Innate Immune System Pathway 1119319!!Legionellosis Pathway 469198