catalog number :
MBS219362
products full name :
MOUSE ANTI V5-TAG:FITC
products short name :
[V5-TAG]
products name syn :
[PK-TAG]
products gene name :
[V5-TAG]
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1mg/ml
storage stability :
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS), Immunofluorescence (IF)
app notes :
Flow Cytometry: Neat -1/5
image1 heading :
Testing Data
other info1 :
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Preservative Stabilisers: 0.09% Sodium Azide (NaN 3 ). 1% Bovine Serum Albumin. Buffer solution: Phosphate buffered saline. Target Species: Viral
other info2 :
Immunogen: A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
products description :
Mouse anti V5-Tag antibody, clone SV5-Pk5 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).