antibody

MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5

MBS219171

0.025 mg

295 USD

monoclonal

mouse

nonconjugated

25-May

Antibody

MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5

ESTROGEN RECEPTOR BETA 5

estrogen receptor beta isoform 2; Estrogen receptor beta; estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2; estrogen receptor 2 (ER beta); Nuclear receptor subfamily 3 group A member 2

ESR2; ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA; ESTRB; NR3A2; ER-beta

ESR2_HUMAN

Monoclonal

IgG1

25-May

Mouse

495

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Paraffin*, Western Blot (WB)

Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Perservative Stabilisers: 0.1% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Histology Positive Control Tissue: Normal colon or colon carcinoma. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5)(Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008). Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied, see Wong, N. et al. for details. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient's prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009).

94538325

NP_001035365.1

NM_001040275.1

Q92731

53,209 Da

Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Integrated Pancreatic Cancer Pathway (711360); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Ovarian Infertility Genes Pathway (198801); Plasma Membrane Estrogen Receptor Signaling Pathway (138009); Prolactin Signaling Pathway (814182)

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 14q23.2. Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus. Molecular Function: ligand-dependent nuclear receptor activity; receptor antagonist activity; protein binding; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; estrogen response element binding; transcription factor activity; steroid binding. Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; ovarian follicle development; cell-cell signaling; regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; steroid hormone mediated signaling; gene expression; brain development; negative regulation of cell growth; negative regulation of epithelial cell proliferation

0.025 mg

295 USD