catalog number :
MBS218961
products full name :
CHIMAERIC HUMAN IgM ANTI NP
products short name :
IgM ANTI NP
specificity :
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.
form :
Tissue Culture Supernatant - liquid
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
ELISA (EIA)
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
other info2 :
Immunogen: Hapten, 4-hydroxy-3-nitrophenylacetyl (NP). Fusion Partners: Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.