MOUSE ANTI HUMAN CD47:Low Endotoxin | MyBioSource MBS217274 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD47:Low Endotoxin

catalog :

MBS217274

quantity :

0.5 mg

price :

550 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1/1A4

reactivity :

human

application :

immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS217274

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD47:Low Endotoxin

products short name :

CD47

other names :

leukocyte surface antigen CD47 isoform 1; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; integrin associated protein; integrin-associated protein; CD47 molecule; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47

products gene name :

CD47

other gene names :

CD47; CD47; IAP; OA3; MER6; MER6; IAP

uniprot entry name :

CD47_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

1/1A4

host :

Mouse

sequence length :

323

form :

Low Endotoxin. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100

other info1 :

Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Immunogen: Human erythrocytes. Histology Positive Control Tissue: Human Tonsil. Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD47 antibody, clone 1/1A4 recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1. Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells (Waclavicek et al. 1993).

ncbi gi num :

4502673

ncbi acc num :

NP_001768.1

ncbi gb acc num :

NM_001777.3

uniprot acc num :

Q08722

ncbi mol weight :

33,845 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Integrins In Angiogenesis Pathway (137999); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q13.1-q13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response

size1 :

0.5 mg

price1 :

550 USD

more info or order :

MyBioSource product webpage