catalog number :
MBS216696
products full name :
RAT ANTI MOUSE CD205:FITC
products short name :
CD205
other names :
lymphocyte antigen 75; Lymphocyte antigen 75; lymphocyte antigen 75; ly-75; lymphocyte antigen 75; DEC-205; CD_antigen: CD205
products gene name :
CD205
other gene names :
Ly75; Ly75; CD205; DEC205; DEC-205; Cd205; Ly-75
uniprot entry name :
LY75_MOUSE
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
other info2 :
Immunogen: Mouse lymph node stroma. Buffer Solution: Phosphate buffered saline. Target Species: Mouse
products description :
Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a 205kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles. The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed. DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.
ncbi acc num :
NP_038853.2
ncbi gb acc num :
NM_013825.3
ncbi mol weight :
197,352 Da