MyBioSource

antibody

RAT ANTI MOUSE CD169:FITC

MBS216655

0.025 mg

260 USD

monoclonal

rat

FITC

mouse

flow cytometry, FACS

MBS216655

Antibody

RAT ANTI MOUSE CD169:FITC

CD169

sialoadhesin; Sialoadhesin; sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1; sialic acid binding Ig-like lectin 1, sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD_antigen: CD169

CD169

Siglec1; Siglec1; Sn; Cd169; Siglec-1; Sa; Sn; SER; Siglec-1

SN_MOUSE

Monoclonal

IgG2a

3D6.112

Rat

1701

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG concentration 0.1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution: Phosphate buffered saline. Target Species: Mouse

Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).

226958332

NP_035556.3

NM_011426.3

Q62230

172,769 Da

Cell Adhesion Molecules (CAMs) Pathway 83266!!Cell Adhesion Molecules (CAMs) Pathway 480

0.025 mg

260 USD