MOUSE ANTI RAT CD163:Biotin | MyBioSource MBS216352 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI RAT CD163:Biotin

catalog :

MBS216352

quantity :

0.1 mg

price :

550 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

ED2

reactivity :

human

application :

flow cytometry, FACS

product information

catalog number :

MBS216352

products type :

Antibody

products full name :

MOUSE ANTI RAT CD163:Biotin

products short name :

CD163

other names :

CD163; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen; CD163 molecule; Hemoglobin scavenger receptor; CD_antigen: CD163Soluble CD163; sCD163

products gene name :

CD163

other gene names :

CD163; CD163; M130; MM130; M130; sCD163

uniprot entry name :

C163A_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

ED2

host :

Mouse

sequence length :

1116

form :

Biotin. Purified IgG conjugated to Biotin - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul . Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Rat

products description :

Mouse anti rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.

ncbi gi num :

5107945

ncbi acc num :

CAB45233.1

uniprot acc num :

Q86VB7

ncbi mol weight :

124,958 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

ncbi summary :

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: integral to plasma membrane; extracellular region; plasma membrane. Molecular Function: protein binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; acute-phase response

size1 :

0.1 mg

price1 :

550 USD