catalog number :
MBS216342
products full name :
CHIMERIC HUMAN IgE ANTI NP
products short name :
CHIMERIC HUMAN IgE ANTI NP
form :
Tissue culture supernatant - liquid.
concentration :
IgE concentration 0.02 - 0.05 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
ELISA (EIA), Functional Assays (FN)*
app notes :
ELISA: Minimum Dilution: 1/20; Maximum Dilution: 1/100;. Functional Assays: Application Note: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide
other info2 :
Immunogen: Hapten, 4-hydroxy-3-nitrophenylacetyl (NP). Fusion Partners: Plasmids containing chimeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line. Buffer Solution: TRIS buffered saline. Target Species: Human
products description :
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.