antibody

MOUSE ANTI RICIN B CHAIN

MBS216336

0.2 mg

430 USD

monoclonal

mouse

nonconjugated

[RB999]

western blot, ELISA, enzyme immunoassay

Antibody

MOUSE ANTI RICIN B CHAIN

[RICIN]

[Ricin; Ricin; Ricin precursor; rRNA N-glycosidase]

[RCOM_2159910]

RICI_RICCO

Monoclonal

IgG1

[RB999]

Mouse

576

RICIN B CHAIN

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Plants

Mouse anti Ricin B Chain antibody, clone RB999 recognizes the B chain of the ricin toxin (RCA60), a 60kDa glycoprotein. It has no cross-reactivity with agglutinin RCA120. The ricin toxin consists of two chains, A and B, covalently linked by a disulfide bond. The A chain acts as a glycosidase that removes an adenine residue from the 28S rRNA. The area from which the adenine is removed is essential for binding of elongation factors, destroying it leaves the ribosome inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, much faster than the cell can replace them, meaning that a single ricin A chain can kill a cell. The B chain binds to complex carbohydrates on the cell surface to facilitate entry of the A chain into the cell. Without the B chain, the A chain has very low toxicity. Ricin is easily extracted from the castor bean (Ricinus communis), and the lethal dose for humans is very small. It is toxic when inhaled, ingested or injected and there is no known antidote, although a vaccine has been developed. Treatment is symptomatic and supportive, with long term organ damage likely in survivors. The ease of extraction and distribution makes it a potential candidate for biological warfare although it is less stable than other agents, such as anthrax.

255588578

XP_002534649.1

XM_002534603.1

P02879

64,091 Da

Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).

0.2 mg

430 USD