MOUSE ANTI HUMAN CD45 | MyBioSource MBS216311 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD45

catalog :

MBS216311

quantity :

0.025 mg

price :

140 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

western blot, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS216311

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD45

products short name :

CD45

other names :

receptor-type tyrosine-protein phosphatase C isoform 1; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45

products gene name :

CD45

other gene names :

PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45; L-CA

uniprot entry name :

PTPRC_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

135-4C5

host :

Mouse

sequence length :

1,143

specificity :

This item recognises the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Clone 135-4C5 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Western Blot: This item detects a band/s of approximately 180kDa, 200kDa, 210kDa and 220kDa in B-cell lysates. Immunohistology - Frozen: Maximum Dilution: 1/200. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100. Immunohistology - Paraffin: Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Purified IgG prepared by affinity chromatography on Protein G

other info2 :

Histology Positive Control Tissue: Human tonsil. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

MBS214488 recognises the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Clone 135-4C5 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes.

ncbi gi num :

392307007

ncbi acc num :

NP_002829.3

ncbi gb acc num :

NM_002838.4

uniprot acc num :

P08575

ncbi mol weight :

130,898 Da

ncbi pathways :

Adaptive Immune System Pathway 366160!!Axon Guidance Pathway 105688!!B Cell Receptor Signaling Pathway 198909!!BCR Signaling Pathway 138058!!CXCR4-mediated Signaling Events Pathway 137910!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Developmental Biology Pathway 477129!!EPO Receptor Signaling Pathway 198882!!Fc Gamma R-mediated Phagocytosis Pathway 114228

ncbi summary :

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

size :

0.025 mg

price :

140 USD

more info or order :

MyBioSource product webpage