MOUSE ANTI HUMAN CD172a:FITC | MyBioSource MBS216216 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD172a:FITC

catalog :

MBS216216

quantity :

0.025 mg

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

reactivity :

human

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS216216

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD172a:FITC

products short name :

CD172a

other names :

tyrosine-protein phosphatase non-receptor type substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1; CD172 antigen-like family member A; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; inhibitory receptor SHPS-1; macrophage fusion receptor; myd-1 antigen; tyrosine phosphatase SHP substrate 1; signal-regulatory protein alpha; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD_antigen: CD172a

products gene name :

CD172a

other gene names :

SIRPA; SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3

uniprot entry name :

SHPS1_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

15-414

host :

Mouse

sequence length :

503

form :

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

concentration :

IgG concentration 0.1mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Immunogen: Monocyte-derived dendritic cells. Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

ncbi gi num :

91105764

ncbi acc num :

NP_001035111.1

ncbi gb acc num :

NM_001040022.1

uniprot acc num :

P78324

ncbi mol weight :

54,852 Da

ncbi pathways :

BDNF Signaling Pathway 712093!!Cardiac Progenitor Differentiation Pathway 712094!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Cell-Cell Communication Pathway 477132!!Hemostasis Pathway 106028!!IL-1 Signaling Pathway 198908!!Osteoclast Differentiation Pathway 193147!!Osteoclast Differentiation Pathway 193096!!Prolactin Signaling Pathway 672462!!Signal Regulatory Protein (SIRP) Family Interactions Pathway 187209

ncbi summary :

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]

size :

0.025 mg

price :

165 USD

more info or order :

MyBioSource product webpage