catalog number :
MBS215901
products full name :
MOUSE ANTI PIG CD169
products short name :
CD169
other names :
sialoadhesin; Sialoadhesin; sialoadhesin; Sialic acid-binding Ig-like lectin 1; Sialic acid-binding Ig-like lectin 1; Siglec-1; p210
products gene name :
CD169
other gene names :
SIGLEC-1; SIGLEC1; SA; SN; pSn; p210; SIGLEC1; SA; SN; pSn; Siglec-1
uniprot entry name :
SN_PIG
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Histology Positive Control Tissue: Porcine spleen. Buffer Solution: Phosphate buffered saline. Target Species: Pig
products description :
Mouse anti Pig CD169, clone 3B11/11 Is specific for porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003). Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer's patches. Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011). Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al).
ncbi acc num :
NP_999511.1
ncbi gb acc num :
NM_214346.1
ncbi mol weight :
184,912 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway 84481!!Cell Adhesion Molecules (CAMs) Pathway 480