antibody

RAT ANTI MOUSE CD206

MBS215657

0.25 mg

475 USD

monoclonal

rat

nonconjugated

[MR5D3]

immunocytochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - frozen section

Antibody

RAT ANTI MOUSE CD206

[CD206]

[macrophage mannose receptor 1; Macrophage mannose receptor 1; macrophage mannose receptor 1; MMR; mannose receptor, C type 1; CD_antigen: CD206]

[CD206]

[Mrc1; Mrc1; MR; CD206; AW259686; MMR]

MRC1_MOUSE

Monoclonal

IgG2a

[MR5D3]

Rat

Mouse

1456

Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002). The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005). Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Purified IgG - liquid

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Flow Cytometry (FC/FACS)*, Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Immunofluorescence (IF). *CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.

FC/FACS: 1/10 - 1/20; Use 10ul of the suggested working dilution to label 10 6 cell in 100ul. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.

Buffer Solution: Phosphate buffered saline. Preservative Stabilisers: 0.09% Sodium Azide. Carrier Free: Yes. Approx. Protein Concentrations: IgG concentration 1.0 mg/ml. Immunogen: Chimaeric CRD4-7-Fc protein. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line. Shelf Life: 18 months from date of dispatch.

224967062

NP_032651.2

NM_008625.2

Phagosome Pathway (153914); Phagosome Pathway (153859); Tuberculosis Pathway (213781); Tuberculosis Pathway (213743)

MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc. Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; endosome membrane; endosome. Molecular Function: mannose binding; transmembrane receptor activity; receptor activity; carbohydrate binding. Biological Process: receptor-mediated endocytosis; endocytosis; signal transduction

0.25 mg

475 USD