antibody

MOUSE ANTI DOG CD45

MBS215442

2 mL

420 USD

monoclonal

mouse

nonconjugated

CA12.10C12

flow cytometry, FACS

Antibody

MOUSE ANTI DOG CD45

CD45

CD45, partial; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45

CD45

PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45; L-CA

PTPRC_HUMAN

Monoclonal

IgG1

CA12.10C12

Mouse

72

Tissue Culture Supernatant - liquid

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Immunohistology - Paraffin: Application Note: This product requires heat mediated antigen retrieval (citrate buffer, pH 6.0).

Perservative Stabilisers: 0.1% Sodium Azide

Target Species: Dog

Mouse anti Dog CD45 antibody, clone CD12.10C12recognises the canine homologue of CD45, clustered at the firstInternational Canine Leukocyte Workshop (Cobbold et al. 1994). Clone CA12.10C12 recognises a glycoprotein of approximately 200kD which is expressed by cells of haemapoietic origin.

44242008

AAS46946.1

130,898 Da

Adaptive Immune System Pathway (366160); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); EPO Receptor Signaling Pathway (198882); Fc Gamma R-mediated Phagocytosis Pathway (114228)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.3.48; Receptor protein phosphatase, tyrosine. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft. Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; stem cell development; B cell receptor signaling pathway; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To

2 mL

420 USD