antibody

RAT ANTI MOUSE CD115

MBS215326

0.1 mg

335 USD

monoclonal

rat

nonconjugated

[604B5 2E11]

flow cytometry, FACS

Antibody

RAT ANTI MOUSE CD115

[CD115]

[macrophage colony-stimulating factor 1 receptor; Macrophage colony-stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; c-fms; proto-oncogene c-Fms; proto-oncogene fms; colony stimulating factor 1 receptor; CSF-1 receptor (EC:2.7.10.1); CSF-1-R; CSF-1R; M-CSF-R; Proto-oncogene c-Fms; CD_antigen: CD115]

[CD115]

[Csf1r; Csf1r; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359; Csfmr; Fms; CSF-1-R; CSF-1R; M-CSF-R]

CSF1R_MOUSE

Monoclonal

IgG1

[604B5 2E11]

Rat

977

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 10 6 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50

Testing Data

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by ion exchange chromatography

Immunogen: Raw 264 cells. Fusion Partners: Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Mouse

Rat anti Mouse CD115 antibody, clone 604B5 2E11 recognizes the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Rat anti Mouse CD115 antibody, clone 604B5 2E11 has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.

126723423

NP_001032948.2

NM_001037859.2

P09581

109,179 Da

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Osteoclast Differentiation Pathway (193149); Osteoclast Differentiation Pathway (193096); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979)

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors. Protein type: EC 2.7.10.1; Kinase, protein; Oncoprotein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; PDGFR family. Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; intracellular; receptor complex. Molecular Function: transferase activity; macrophage colony stimulating factor receptor activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; cytokine binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding; protein kinase activity. Biological Process: regulation of bone resorption; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; immune system process; positive regulation of osteoclast differentiation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; phosphatidylinositol metabolic process; positive regulation of protein amino acid phosphorylation; intercellular junction maintenance; inflammatory response; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

0.1 mg

335 USD