antibody

MOUSE ANTI HUMAN CD99

MBS215284

0.025 mg

140 USD

monoclonal

mouse

nonconjugated

DN16

western blot, flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD99

CD99

CD99 antigen isoform b; CD99 antigen; CD99 antigen; E2 antigen; MIC2 (monoclonal antibody 12E7); T-cell surface glycoprotein E2; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; surface antigen MIC2; CD99 molecule; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD_antigen: CD99

CD99

CD99; CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X; MIC2; MIC2X; MIC2Y

CD99_HUMAN

Monoclonal

IgG1

DN16

Mouse

169

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Perservative Stabilisers: 0.1% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by DEAE - dextran chromatography

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002). CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells. Functionally CD99 has been found to be involved in cellular adhesion/aggregation (Krisanaprakornkit et al. 2013) and apoptosis (Sciandra et al. 2014).

171543879

NP_001116370.1

NM_001122898.1

P14209

17,128 Da

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3. Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane. Biological Process: cell adhesion

0.025 mg

140 USD