MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA | MyBioSource MBS215228 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA

catalog :

MBS215228

quantity :

0.1 ml

price :

195 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS215228

products type :

Antibody

products full name :

MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA

products short name :

ESTROGEN RECEPTOR ALPHA

other names :

estrogen receptor isoform 1; Estrogen receptor; estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1; estrogen receptor 1; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

other gene names :

ESR1; ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1; ESR; NR3A1; ER

uniprot entry name :

ESR1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

6F11

host :

Mouse

sequence length :

595

form :

Tissue Culture Supernatant - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen*, Immunohistology Paraffin*

app notes :

Immunohistology - Frozen: Minimum Dilution: 1/40; Maximum Dilution: 1/60; Application Note: The use of Zamboni 's fixative is recommended for best results . Immunohistology - Paraffin: Minimum Dilution: 1/40; Maximum Dilution: 1/80; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide

other info2 :

Immunogen: Recombinant human estrogen receptor (alpha form). Histology Positive Control Tissue: Breast carcinoma. Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line. Target Species: Human

products description :

Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERalpha is a ~65kDa steroid hormone receptor containing an N-terminal AF-1).ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an AF-2 domain. ERalpha binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta (ERbeta) The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al. 2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargava et al. 2012). Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERalpha on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERalpha in breast cancer tissues (Ambroise et al. 2011).

ncbi gi num :

62821794

ncbi acc num :

NP_000116.2

ncbi gb acc num :

NM_000125.3

uniprot acc num :

P03372

ncbi mol weight :

35,547 Da

ncbi pathways :

Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213307!!Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213276!!Estrogen Signaling Pathway 799177!!Estrogen Signaling Pathway 198863!!Estrogen Signaling Pathway 799197!!FOXA1 Transcription Factor Network Pathway 137979!!FOXM1 Transcription Factor Network Pathway 137935!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Integrated Breast Cancer Pathway 219801

ncbi summary :

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

size :

0.1 ml

price :

195 USD

more info or order :

MyBioSource product webpage