MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin | MyBioSource MBS215223 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin

catalog :

MBS215223

quantity :

0.25 mg

price :

560 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

[CC302]

reactivity :

cow

application :

ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.

image 2 :

Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.

image 3 :

Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 ug/ml polyI:C, 0.5 ug/ml R-848 or 5 ug/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.From: Ferret-Bernard S, Remot A, Lacroix-Lamand© S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.

product information

catalog number :

MBS215223

products type :

Antibody

products full name :

MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin

products short name :

[IFN GAMMA]

other names :

[interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; boIFNG; interferon gamma type 2; interferon-gamma; interferon, gamma<]

other gene names :

[IFNG; IFNG; BoIFNG; IFN-gamma]

uniprot entry name :

IFNG_BOVIN

clonality :

Monoclonal

isotype :

IgG1

clone :

[CC302]

host :

Mouse

sequence length :

166

form :

Biotin. Purified IgG conjugated to Biotin - liquid

concentration :

IgG concentration 0.5 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Flow cytometry (FC/FACS)*

app notes :

ELISA: Biotinylated mouse anti bovine IFN gamma, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFN gamma, clone CC330, as the capture reagent and recombinant bovine IFN gamma as the standard. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ELISA: Maximum Dilution: 5ug/ml. Flow Cytometry: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Application Note: Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm

image1 heading :

Testing Data

image2 heading :

Testing Data

image3 heading :

Testing Data

image4 heading :

Testing Data

image4 description :

Published customer image: gamma delta T cells require TNF-alpha to protect against B. abortus infection. C57BL/6 mice treated with anti-TCR gamma delta mAb or hamster IgG on day -1 and day 3 post-infection with 5x104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-alpha on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean +/- SEM of 10 mice/group is depicted; *P

image5 heading :

Testing Data

image5 description :

Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Bovine

products description :

Mouse anti Bovine IFN gamma antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferitive properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20kDa. IFN gamma is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000). Mouse anti bovine gamma inerferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Clone CC302 has been successfully used for the evaluation of gamma interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using ELISA.

ncbi gi num :

31982949

ncbi acc num :

NP_776511.1

ncbi gb acc num :

NM_174086.1

uniprot acc num :

P07353

ncbi mol weight :

19,393 Da

ncbi pathways :

African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (84313); Antigen Processing And Presentation Pathway (485); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795)

uniprot summary :

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

size1 :

0.25 mg

price1 :

560 USD

more info or order :

MyBioSource product webpage