catalog number :
MBS215211
products full name :
MOUSE ANTI DOG CD3
products short name :
CD3
other names :
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3E antigen, epsilon polypeptide (TiT3 complex); CD_antigen: CD3e
other gene names :
CD3E; CD3E; N/A
uniprot entry name :
CD3E_CANFA
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood . Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Dog
products description :
Mouse anti Dog CD3 antibody, clone CA17.2A12 recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).
ncbi acc num :
NP_001003379.1
ncbi gb acc num :
NM_001003379.1
ncbi mol weight :
22,642 Da
ncbi pathways :
Adaptive Immune System Pathway 1127763!!Chagas Disease (American Trypanosomiasis) Pathway 147812!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Costimulation By The CD28 Family Pathway 1127769!!Downstream TCR Signaling Pathway 1127768!!Generation Of Second Messenger Molecules Pathway 1127767!!HTLV-I Infection Pathway 373907!!HTLV-I Infection Pathway 373889!!Hematopoietic Cell Lineage Pathway 84128!!Hematopoietic Cell Lineage Pathway 489