MOUSE ANTI HUMAN CD20:FITC | MyBioSource MBS215157 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD20:FITC

catalog :

MBS215157

quantity :

0.025 mg

price :

165 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

2H7

reactivity :

human

application :

flow cytometry, FACS

product information

catalog number :

MBS215157

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD20:FITC

products short name :

CD20

other names :

B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20

products gene name :

CD20

other gene names :

MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20

uniprot entry name :

CD20_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

2H7

host :

Mouse

sequence length :

297

form :

FITC. Purified IgG conjugated to Fluorescein isothiocyanate isomer 1 (FITC) - liquid.

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood . Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells. The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

ncbi gi num :

23110987

ncbi acc num :

NP_068769.2

ncbi gb acc num :

NM_021950.3

uniprot acc num :

P11836

ncbi mol weight :

14,581 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family. Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane. Molecular Function: epidermal growth factor receptor binding. Biological Process: B cell proliferation; humoral immune response. Disease: Immunodeficiency, Common Variable, 5

size1 :

0.025 mg

price1 :

165 USD