antibody

MOUSE ANTI HUMAN MAST CELL TRYPTASE

MBS215015

0.02 mg

175 USD

monoclonal

mouse

nonconjugated

AA1

immunocytochemistry, enzyme immunoassay

Antibody

MOUSE ANTI HUMAN MAST CELL TRYPTASE

MAST CELL TRYPTASE

tryptase alpha/beta-1; Tryptase alpha/beta-1; tryptase alpha/beta-1; mast cell alpha II tryptase; mast cell beta I tryptase; tryptase I; tryptase alpha II; tryptase alpha-1; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase-1; tryptase-I; tryptase-III; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1

TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1

TRYB1_HUMAN

Monoclonal

IgG1

AA1

Mouse

275

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin*

Immunohistology - Paraffin: Maximum Dilution: 1/10,000; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Histology Positive Control Tissue: Tonsil. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human Mast Cell Tryptase, clone AA1 recognizes human mast cell tryptase, both alpha and beta isoforms.Mouse anti Mast cel tryptase, clone AA! is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Walls et al. 1990). Tryptases are the products of a number of genes and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997).

13775595

NP_003285.2

NM_003294.3

29,533 Da

Activation Of Matrix Metalloproteinases Pathway 576264!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

0.02 mg

175 USD