MyBioSource

antibody

MOUSE ANTI PIG CD14:FITC

MBS214839

0.1 mg

610 USD

monoclonal

mouse

FITC

cow

FACS, flow cytometry

MBS214839

Antibody

MOUSE ANTI PIG CD14:FITC

CD14

CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein; CD14 molecule<; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14

CD14

CD14; CD14; N/A

CD14_BOVIN

Monoclonal

IgG2b

MIL2

Mouse

373

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG concentration 0.1mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Pig

Mouse anti Pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Clone MIL2 immunoprecipitates a protein of ~50kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thacker et al. 2001). Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone T K4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickova et al. 2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersen et al.2007 and Sanz et al. 2007).

2274810

BAA21517.1

39,667 Da

Activated TLR4 Signalling Pathway 1119352!!Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway 1119338!!Amoebiasis Pathway 167315!!Amoebiasis Pathway 167191!!Hematopoietic Cell Lineage Pathway 84317!!Hematopoietic Cell Lineage Pathway 489!!IKK Complex Recruitment Mediated By RIP1 Pathway 1119339!!Immune System Pathway 1119286!!Innate Immune System Pathway 1119319!!Legionellosis Pathway 469198

0.1 mg

610 USD