RAT ANTI MOUSE CD8 | MyBioSource MBS214794 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI MOUSE CD8

catalog :

MBS214794

quantity :

0.025 mg

price :

175 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[YTS105.18]

reactivity :

mouse

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

image 2 :

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power

image 3 :

Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026)

product information

catalog number :

MBS214794

products type :

Antibody

products full name :

RAT ANTI MOUSE CD8

products short name :

[CD8 ALPHA]

products name syn :

[CD8 Alpha; LY-2; Lyt2; Lyt-2]

other names :

[T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2; CD8 antigen, alpha chain; T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a]

products gene name :

[CD8]

other gene names :

[Cd8a; Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; Lyt-2; Lyt2]

uniprot entry name :

CD8A_MOUSE

clonality :

Monoclonal

isotype :

IgG2a

clone :

[YTS105.18]

host :

Rat

reactivity :

Mouse

sequence length :

247

specificity :

Rat anti Mouse CD8, clone YTS105.18 recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo and induces transplantation tolerance in combination with CD4 antibodies.

form :

Purified IgG - liquid

concentration :

IgG concentration 1 mg/ml

storage stability :

Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology-Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Suggested Dilution 1/50-1/100

image1 heading :

Testing Data

image2 heading :

Testing Data

image3 heading :

Testing Data

image4 heading :

Testing Data

image4 description :

Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power

image5 heading :

Testing Data

image5 description :

Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C

other info1 :

Target Species: Mouse. Immunogen: Mouse spleen cells. Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Carrier Free: Yes

other info2 :

Buffer Solution: Phosphate buffered saline. Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line

ncbi gi num :

124487437

ncbi acc num :

NP_001074579.1

ncbi gb acc num :

NM_001081110.2

ncbi pathways :

Adaptive Immune System Pathway (1110669); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1110668); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1110695); Primary Immunodeficiency Pathway (83318)

uniprot summary :

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; protein kinase binding. Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus

size1 :

0.025 mg

price1 :

175 USD

more info or order :

MyBioSource product webpage