antibody

MOUSE ANTI HUMAN CA125

MBS214597

0.2 mg

430 USD

monoclonal

mouse

nonconjugated

[X75]

western blot, ELISA

Antibody

MOUSE ANTI HUMAN CA125

[CA125]

[mucin-16; Mucin-16; mucin-16; CA125 ovarian cancer antigen; ovarian cancer-related tumor marker CA125; ovarian carcinoma antigen CA125; mucin 16, cell surface associated; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125]

[CA125]

[MUC16; MUC16; CA125; CA125; MUC-16; CA-125]

MUC16_HUMAN

Monoclonal

IgG1

[X75]

Mouse

Human

Mouse anti Human CA125 antibody, clone X75 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X75 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X75 has been used successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the measurement of CA125 antigen in human plasma samples.

Purified IgG prepared by ion exchange chromatography

Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

ELISA, Western Blot (WB)

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Target Species: Human. Immunogen: CA125 antigen purified from human ovarian carcinoma.

Buffer Solution: TRIS buffered saline. Preservative Stabilizers: 0.09% Sodium Azide (NaN 3 ). Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

83367077

NP_078966.2

NM_024690.2

Q8WXI7

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Protein type: Cell adhesion; Membrane protein, peripheral; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification

0.2 mg

430 USD