antibody

MOUSE ANTI HUMAN TROPONIN I (CARDIAC/PHOSPHORYLATED)

MBS214525

0.2 mg

400 USD

monoclonal

mouse

nonconjugated

1G11

human, rat, dog, cow

western blot, ELISA, enzyme immunoassay

Antibody

MOUSE ANTI HUMAN TROPONIN I (CARDIAC/PHOSPHORYLATED)

TROPONIN I

troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac); Cardiac troponin I

TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; TNNC1

TNNI3_HUMAN

Monoclonal

IgG2b

1G11

Mouse

Bovine, Cat, Dog, Goat, Pig, Rat

210

Purified. Purified IgG - liquid

IgG concentration 1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)*

Western Blotting: Application Note: For the detection of phosphoproteins, phosphatase inhibitors such as 10mM Sodium Fluoride and 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogen: Phosphorylated form of human cardiac Troponin I. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human Troponin I (Cardiac/Phosphorylated) antibody, clone 1G11 recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kDa subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Mouse anti Human Troponin I (Cardiac/Phosphorylated) antibody, clone 1G11 recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I.

151101270

NP_000354.4

NM_000363.4

P19429

24,008 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: sarcomere; troponin complex; cytosol. Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding. Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding. Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a

0.2 mg

400 USD