MOUSE ANTI HUMAN TROPONIN I (CARDIAC/DEPHOSPHORYLATED) | MyBioSource MBS214524 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN TROPONIN I (CARDIAC/DEPHOSPHORYLATED)

catalog :

MBS214524

quantity :

0.2 mg

price :

400 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

22B11

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS214524

products type :

Antibody

products full name :

MOUSE ANTI HUMAN TROPONIN I (CARDIAC/DEPHOSPHORYLATED)

products short name :

TROPONIN I

other names :

troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac); Cardiac troponin I

other gene names :

TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; TNNC1

uniprot entry name :

TNNI3_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

22B11

host :

Mouse

reactivity :

Pig (Weak)

sequence length :

210

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Western Blot (WB)

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Immunogen: Dephosphorylated form of human cardiac Troponin I. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human Troponin I (Cardiac/Dephosphorylated) antibody, clone 22B11 recognizes the dephosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kDa subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Mouse anti Human Troponin I (Cardiac/Dephosphorylated) antibody, clone 22B11 recognizes the dephosphorylated form of cTnI. It specifically recognizes amino acid residues 20-24 on cTnI. The antibody will recognize free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. MBS214524 does not cross react with phosphorylated cTnI or with skeletal TnI.

ncbi gi num :

151101270

ncbi acc num :

NP_000354.4

ncbi gb acc num :

NM_000363.4

uniprot acc num :

P19429

ncbi mol weight :

24,008 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

ncbi summary :

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

uniprot summary :

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: sarcomere; troponin complex; cytosol. Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding. Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding. Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a

size1 :

0.2 mg

price1 :

400 USD

more info or order :

MyBioSource product webpage