antibody

MOUSE ANTI HUMAN BAX (N-TERMINAL)

MBS214489

0.1 mg

550 USD

monoclonal

mouse

nonconjugated

2D2

human, cow

western blot, ELISA, immunocytochemistry, immunoprecipitation, enzyme immunoassay

Antibody

MOUSE ANTI HUMAN BAX (N-TERMINAL)

BAX

apoptosis regulator BAX isoform 1; Apoptosis regulator BAX; apoptosis regulator BAX; BCL2-associated X protein omega; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; bcl-2-like protein 4; bcl2-L-4; BCL2-associated X protein; Bcl-2-like protein 4; Bcl2-L-4

BAX

BAX; BAX; BCL2L4; BCL2L4; Bcl2-L-4

BAX_HUMAN

Monoclonal

IgG1

2D2

Mouse

African green monkey, Bovine

221

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Western Blot: This item detects a band of approximately 23kDa in COLO320 DM cell lysates. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml. Immunofluorescence: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml. Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human BAX antibody, clone 2D2 recognizes an epitope within the N-terminal (NT) region of human apoptosis regulator BAX, also known as Bcl-2-like protein 4. BAX is a pro-apoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor Bcl-2 or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C, activation of caspase-3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma (Brimmell et al. 1998). Mouse anti Human BAX antibody, clone 2D2 has been successfully used for the immunoprecipitation of bovine BAX from thymus (Basa ez et al. 1999).

612149787

NP_001278357.1

NM_001291428.1

Q07812

19,718 Da

Activation, Translocation And Oligomerization Of BAX Pathway 105665!!AhR Pathway 755436!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

0.1 mg

550 USD