MOUSE ANTI HUMAN BUB1 | MyBioSource MBS214428 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN BUB1

catalog :

MBS214428

quantity :

0.05 mg

price :

500 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14H5

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

product information

catalog number :

MBS214428

products type :

Antibody

products full name :

MOUSE ANTI HUMAN BUB1

products short name :

BUB1

other names :

mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 3; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; BUB1 mitotic checkpoint serine/threonine kinase; BUB1A

products gene name :

BUB1

other gene names :

BUB1; BUB1; BUB1A; BUB1L; hBUB1; BUB1L; hBUB1

uniprot entry name :

BUB1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

14H5

host :

Mouse

sequence length :

1028

specificity :

This item detects Bub1, a protein kinase involved in spindle checkpoint function during mitosis. Bub1 co-operates with Aurora B kinase to inhibit Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align on the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelity mitotic chromosome segregation. Bub1 is also required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshin protein, and metaphase congression. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunofluorescence (IF)*, Immunoprecipitation (IP), Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 160kDa in HeLa cell lysates. Immunofluorescence: Minimum Dilution: 1/500; Maximum Dilution: 1/2000; Application Note: Use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH6.8, or 100% methanol at -20 o C. Permeabilise cells with 0.5% Triron X-100. . Immunoprecipitation: Maximum Dilution: 1/200. Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/2000

other info1 :

Perservative Stabilisers: 0.01% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Immunogen: Recombinant protein corresponding to amino acids 281-419 of human Bub1 protein. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

MBS214428 detects Bub1, a protein kinase involved in spindle checkpoint function during mitosis. Bub1 co-operates with Aurora B kinase to inhibit Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align on the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelity mitotic chromosome segregation. Bub1 is also required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshin protein, and metaphase congression. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer.

ncbi gi num :

519666801

ncbi acc num :

NP_001265546.1

ncbi gb acc num :

NM_001278617.1

uniprot acc num :

O43683

ncbi mol weight :

120,002 Da

ncbi pathways :

Aurora B Signaling Pathway 138080!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!M Phase Pathway 105812!!Mitotic Anaphase Pathway 105818!!Mitotic Metaphase And Anaphase Pathway 730317!!Mitotic Prometaphase Pathway 105815

ncbi summary :

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

size :

0.05 mg

price :

500 USD