MyBioSource

antibody

MOUSE ANTI HUMAN CD289

MBS214287

0.1 mg

400 USD

monoclonal

mouse

western blot, flow cytometry, FACS

MBS214287

Antibody

MOUSE ANTI HUMAN CD289

CD289

toll-like receptor 9; Toll-like receptor 9; toll-like receptor 9; toll-like receptor 9; CD_antigen: CD289

CD289

TLR9; TLR9; CD289

TLR9_HUMAN

Monoclonal

IgG2a

5G5

Mouse

1032

Purified. Purified IgG - liquid

IgG concentration 0.5mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunohistology Paraffin, Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Western Blot: This item detects a band of approximately 170-180 kDa in RAW264 cell lysates. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

Perservative Stabilisers: 0.09% Sodium Azide

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human CD289 antibody, clone 5G5 recognizes human, canine and murine TLR9, otherwise known as CD289. TLR9 is a member of the Toll-like receptor (TLR) family and functions as a receptor for bacterial DNA (CpG-DNA), playing a fundamental role in pathogen recognition and activation of innate immunity.TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. In mouse RAW264 cells TLR9 has been shown to be expressed intracellularly, but not at the cell surface.

8394456

NP_059138.1

NM_017442.3

Q9NR96

114,237 Da

African Trypanosomiasis Pathway 194384!!African Trypanosomiasis Pathway 194323!!Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Herpes Simplex Infection Pathway 377873!!Herpes Simplex Infection Pathway 377865!!IGF1R Signaling Cascade Pathway 730346!!IRS-mediated Signalling Pathway 106426!!IRS-related Events Pathway 106424!!IRS-related Events Triggered By IGF1R Pathway 730348

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

0.1 mg

400 USD