antibody

HAMSTER ANTI NOTCH 1

MBS214228

0.1 mL

770 USD

monoclonal

hamsters

nonconjugated

8G10

mouse, rat

western blot, flow cytometry, FACS

Antibody

HAMSTER ANTI NOTCH 1

NOTCH 1

neurogenic locus notch homolog protein 1; Neurogenic locus notch homolog protein 1; neurogenic locus notch homolog protein 1; Motch A; Notch gene homolog 1; mT14; major type A protein; p300; transmembrane receptor Notch1; notch 1; Motch A; mT14; p300Cleaved into the following 2 chains:Notch 1 extracellular truncation; NEXT; Notch 1 intracellular domain; NICD

NOTCH 1

Notch1; Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik; Motch; Notch 1; NEXT; NICD

NOTC1_MOUSE

Monoclonal

IgG1

8G10

Hamster

Rat

2531

This item specifically recognises Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.

Tissue Culture Supernatant - liquid

Store at -20 degree C only (ship 4 degree C). Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Western Blot (WB)

Perservative Stabilisers: 10ug/ml Ciprofloxacin 20% FCS

Immunogen: A GST fusion protein using the mouse Notch 1 amino acid sequence 1299-1492. Histology Positive Control Tissue: Brain; Lung; Thymus. Target Species: Mouse

MBS214228 specifically recognises Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.

224967065

NP_032740.3

NM_008714.3

Q01705

269,180 Da

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (1110490); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (1111366); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1111370); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1111368); Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway (1111364); Delta-Notch Signaling Pathway (198316); Developmental Biology Pathway (1111057); Disease Pathway (1111319); Dorso-ventral Axis Formation Pathway (114296); Dorso-ventral Axis Formation Pathway (472)

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family. Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Transcription factor; Oncoprotein. Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; cell; cytoplasm; acrosome; integral to membrane; plasma membrane; nucleus; receptor complex. Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; chromatin binding; receptor activity; calcium ion binding; transcription factor activity. Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; regulation of cell migration; negative regulation of BMP signaling pathway; compartment specification; neuron differentiation; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of astrocyte differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of keratinocyte differentiation; embryonic limb morphogenesis; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; cell fate commitment; transcription, DNA-dependent; neuron fate commitment; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; negative regulation of neuron differentiation; branching morphogenesis of a tube; regulation of gene expression; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; anagen; positive regulation of epithelial cell proliferation; foregut morphogenesis; endoderm development; osteoblast fate commitment; transcription from RNA polymerase II promoter; epidermis development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of cell proliferation; regulation of neurogenesis; glial cell differentiation; regulation of transcription, DNA-dependent; astrocyte differentiation; positive regulation of cell proliferation; cardiac muscle morphogensis; forebrain development; heart looping; angiogenesis; regulation of somitogenesis; cell differentiation; positive regulation of BMP signaling pathway; regulation of developmental process; mesenchymal cell development; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; regulation of cell proliferation; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; negative regulation of cell differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; sprouting angiogenesis; auditory receptor cell fate commitment; negative regulation of myoblast differentiation; positive regulation of cell migration; lung development

0.1 mL

770 USD