RAT ANTI SUBSTANCE P | MyBioSource MBS213959 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI SUBSTANCE P

catalog :

MBS213959

quantity :

0.1 mL

price :

470 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[NC1/34]

reactivity :

human

application :

immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Published customer image: A,B. Tachykinin-like immunoreactivity in cells in inflammatory infiltrate of myositis area. Sections of a specimen from a soleus muscle specimen of the 6w group, non-exercised side. In (A), the section had been immunostained with MBS213959, and in (B), the section was immunostained with MBS213959 after preabsorption with tachykinin (SP) (larger area is covered in B than in A). Immunoreactivity is seen in cells in (A), but no reaction is visible in (B). Arrows point at cells of inflammatory infiltrate. The immunoreactions show partly a granular appearance (A). Magnification x315. c,d. Immunostaining with polyclonal and monoclonal tachykinin antibodies. A part of a focal myositis area of a section of a gastrocnemius muscle specimen from the 1w group, exercised side, double-stained for tachykinin using the sc-14104 (C) and MBS213959 (D) antibodies, shown at low magnification. Note that the cells of an inflammatory infiltrate are immunolabelled in both (C) and (D) (arrows), whereas the wall of a small vessel is only immunostained in (C) (asterisks). Magnification x315.From: Song Y, St¥l PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.

image 2 :

Published customer image: Tachykinin-like immunoreactivity in nerve fascicles. Nerve fascicles and parts of nerve fascicles (N) of sections from gastrocnemius (A-C, E, F) and soleus (D) muscle specimens are shown. The sections were processed for demonstration of tachykinin-like immunoreaction (A-E). In (F) the reaction pattern after preabsorption with SP peptide is shown (the region in F corresponds to the region in E). The antibodies applied were sc-14104 (A-C, E) and MBS213959 (D). The specimens were from the control group (A, B, E, F) and from the 6w group, non-exercised side, focal myositis area (C, D). There are immunoreactivities in nerve fascicles in the form of punctuate reactions (arrows A, C-E). These are grouped, as especially seen in (C), but also to some extent in (D). There are no reactions in the nerve fascicle in (B), which is built up of myelinated nerve fibers, and in the control section in (F). MF refers to muscle fibers. Magnification x200 (B, C), x315 (A, D, E, F).From: Song Y, St¥l PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.

product information

catalog number :

MBS213959

products type :

Antibody

products full name :

RAT ANTI SUBSTANCE P

products short name :

[SUBSTANCE P]

products name syn :

[NKA, NKNA, TAC2]

other names :

[protachykinin-1 isoform beta; Protachykinin-1; protachykinin-1; PPT; neurokinin 1; neurokinin 2; neurokinin A; neurokinin alpha; neuromedin L; neuropeptide K; neuropeptide gamma; preprotachykinin; substance K; substance P; tachykinin 2; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma); tachykinin, precursor 1; PPTCleaved into the following 5 chains:Substance P; Neurokinin A; NKAAlternative name(s):Neuromedin L; Substance K]

other gene names :

[TAC1; TAC1; NK2; NPK; NKNA; TAC2; Hs.2563; NKA; NKNA; TAC2; NKA; NPK]

uniprot entry name :

TKN1_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[NC1/34]

host :

Rat

sequence length :

129

specificity :

SUBSTANCE P

form :

Tissue Culture Supernatant - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Immunofluorescence (IF), Immunohistology Paraffin

app notes :

Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/200;. Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections.

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Species Cross Reactivity: Reacts with: Pig, Pigeon, Bichir, Rabbit, Human, Bovine, Guinea Pig, Calanus sp., Cancer sp., Panilurus sp., Homarus sp., Mudpuppy, Lamprey, Crayfish, Zebrafish, Bullfrog, Cockroach, Sheep, Cowbird, Rat, Spadefoot toad, Chicken, Chinchilla, Camel. Based on sequence similarity, is expected to react with: Mammals, Crustacea, Amphibia. N.B. Antibody reactivity and working conditions may vary between species. Perservative Stabilisers: 0.05% Thiomersal

other info2 :

Immunogen: Substance P conjugated to Bovine Serum Albumin. Fusion Partners: Spleen cells from immunised wistar rat were fused with cells of the Ns1/1-Ag 4-1 myeloma cell line.

products description :

Rat anti substance P antibody, clone NC1/34 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.

ncbi gi num :

4507341

ncbi acc num :

NP_003173.1

ncbi gb acc num :

NM_003182.2

uniprot acc num :

P20366

ncbi mol weight :

11,376 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Peptide Ligand-binding Receptors Pathway (106358); SIDS Susceptibility Pathways (198901)

ncbi summary :

This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Substance P is an antimicrobial peptide with antibacterial and antifungal properties. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

uniprot summary :

TAC1: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q21-q22. Cellular Component: extracellular space; cell soma; axon; extracellular region; plasma membrane. Molecular Function: protein binding; substance P receptor binding. Biological Process: tachykinin signaling pathway; negative regulation of heart rate; insemination; response to hormone stimulus; response to morphine; response to lipopolysaccharide; response to pain; sensory perception of pain; positive regulation of saliva secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; cell-cell signaling; positive regulation of acute inflammatory response; positive regulation of lymphocyte proliferation; neuropeptide signaling pathway; regulation of blood pressure; positive regulation of stress fiber formation; positive regulation of action potential; inflammatory response; detection of abiotic stimulus; associative learning; positive regulation of synaptic transmission, cholinergic; positive regulation of synaptic transmission, GABAergic; positive regulation of ossification; long-term memory

size1 :

0.1 mL

price1 :

470 USD

more info or order :

MyBioSource product webpage