antibody

MOUSE ANTI HUMAN CD209:RPE

MBS213786

100 Tests

400 USD

monoclonal

mouse

RPE

MR-1

flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD209:RPE

CD209

CD209 antigen isoform 5; CD209 antigen; CD209 antigen; C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; HIV gpl20-binding protein; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin; CD209 molecule; C-type lectin domain family 4 member L; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; DC-SIGN; DC-SIGN1; CD_antigen: CD209

CD209

CD209; CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1; CLEC4L; DC-SIGN; DC-SIGN1

CD209_HUMAN

Monoclonal

IgG1

MR-1

Mouse

297

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Reconstitution: Reconstitute with 1 ml distilled water. Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. 5% Sucrose. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human CD209 antibody, clone MR-1 recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a 45kD C-type lectin that binds ICAM-3 also known as CD209. CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro.CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's. Clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).

223029380

NP_001138365.1

NM_001144893.1

Q9NNX6

33,874 Da

Measles Pathway (213306); Measles Pathway (213277); Phagosome Pathway (153910); Phagosome Pathway (153859); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]

CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13. Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane. Molecular Function: mannose binding; protein binding; peptide antigen binding; metal ion binding; virion binding; carbohydrate binding. Biological Process: heterophilic cell adhesion; cell-cell recognition; antigen processing and presentation; leukocyte adhesion; virus-host interaction; regulation of T cell proliferation; innate immune response; endocytosis; peptide antigen transport; viral genome replication; virion attachment to host cell surface receptor. Disease: Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

100 Tests

400 USD