MOUSE ANTI HUMAN MYELOPEROXIDASE:RPE | MyBioSource MBS213662 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN MYELOPEROXIDASE:RPE

catalog :

MBS213662

quantity :

100 Tests

price :

450 USD

clonality :

monoclonal

host :

mouse

conjugate :

RPE

clone name :

[2C7]

reactivity :

human, dog

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human MYELOPEROXIDASE:FITC (MBS210766)

image 2 :

Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase (MBS212300) following permeabilisation with Leucoperm

image 3 :

Staining of human bone marrow. Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (MBS215187)

product information

catalog number :

MBS213662

products type :

Antibody

products full name :

MOUSE ANTI HUMAN MYELOPEROXIDASE:RPE

products short name :

[MYELOPEROXIDASE]

other names :

[myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase]

other gene names :

[MPO; MPO; MPO]

uniprot entry name :

PERM_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[2C7]

host :

Mouse

reactivity :

Reacts with Dog. Does not react with Rat. N.B Antibody reactivity and working conditions may vary between species.

sequence length :

745

specificity :

Myeloperoxidase

form :

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

storage stability :

Prior to reconstitution store at 4°C. Following reconstitution store at 4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.

tested application :

Flow cytometry (FC/FACS)*

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

image1 heading :

Testing Data

image2 heading :

Testing Data

image3 heading :

Testing Data

other info1 :

Reconstitution: Reconstitute with 1 ml distilled water. Preservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. 5% Sucrose. Immunogen: Human myeloperoxidase.

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Human. Fusion partners: Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.

products description :

Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond. Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots,and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody,clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA. Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003)

ncbi gi num :

4557759

ncbi acc num :

NP_000241.1

ncbi gb acc num :

NM_000250.1

uniprot acc num :

P05164

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Vitamin B12 Metabolism Pathway (920974); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

uniprot summary :

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.11.2.2; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule. Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding. Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; response to lipopolysaccharide; response to yeast; defense response; response to oxidative stress; defense response to fungus; aging; negative regulation of apoptosis. Disease: Myeloperoxidase Deficiency; Alzheimer Disease

size1 :

100 Tests

price1 :

450 USD

more info or order :

MyBioSource product webpage