catalog number :
MBS213190
products full name :
MOUSE ANTI HUMAN CYTOKERATIN 14
products short name :
CYTOKERATIN 14
other names :
keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; Cytokeratin-14; CK-14; Keratin-14; K14
other gene names :
KRT14; KRT14; K14; NFJ; CK14; EBS3; EBS4; CK-14; K14
uniprot entry name :
K1C14_HUMAN
reactivity :
Dog, Elephant, Lion, Pig
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Immunohistology - Paraffin: Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
other info2 :
Histology Positive Control Tissue: Skin. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011)
ncbi acc num :
NP_000517.2
ncbi gb acc num :
NM_000526.4
ncbi mol weight :
51,561 Da
ncbi pathways :
Corticotropin-releasing Hormone Pathway 920957!!Glucocorticoid Receptor Regulatory Network Pathway 138014
ncbi summary :
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]